Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2 Trade Information

Minimum Order Quantity
10 Pieces

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Days after Acceptance (DA)

Supply Ability
10 Pieces Per Day

Delivery Time
5 Days

Sample Available
No

Packaging Details
Plastic Bottle

Main Export Market(s)
Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa

Main Domestic Market
All India

Certifications
Himedia

About Antibiotic Assay Medium No 2

Antibiotic Assay Medium No. 2 Base Agar is a solid, solidified medium used as a base layer in microbiological assays for determining antibiotic potency. It provides a stable surface for the diffusion of the antibiotic into a seed agar layer inoculated with test organisms. The medium's composition, typically including peptone, yeast extract, and beef extract, provides the necessary nutrients for the growth of the test organisms while also supporting the over-layering of the seed agar. Key Features and Usage:

Base Agar:This medium serves as the foundation for antibiotic assays, particularly those involving agar diffusion techniques like the cylinder-plate method.
Nutrient-Rich:Peptone, yeast extract, and other ingredients provide essential nutrients (carbon, nitrogen, vitamins) for the growth of test organisms, allowing for clear zone measurements.
Solidified Substrate:The agar component provides the solid, stable base layer needed for the diffusion of the antibiotic and the growth of the test organisms in the seed agar layer.
Standardized:The medium is designed to meet specific requirements for microbiological assays, as outlined in pharmacopoeias and standard testing methods.

Preparation and Use:

1. Preparation:The medium is typically prepared by dissolving the dehydrated powder in distilled or deionized water, heating to boiling, and sterilizing by autoclaving.
2. Base Layer:Once sterilized, the medium is poured into Petri dishes to create a solid base layer.
3. Seed Agar:A seed agar layer, inoculated with the test organism, is then carefully layered over the base agar.
4. Diffusion:The antibiotic, in the form of a disc, cylinder, or well, is placed on the base agar, allowing the antibiotic to diffuse into the seeded agar.
5. Zone Measurement:As the antibiotic diffuses, it inhibits the growth of the test organism, creating a clear zone around the antibiotic source. The size of this zone is then measured to determine the antibiotic's potency or activity.

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

Antibiotic Assay Medium No 2

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