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Antibiotic Assay Medium No 3

Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3

Antibiotic Assay Medium No 3 Specification

  • Storage
  • Room Temperature
  • Usage
  • Laboratory
  • Physical Form
  • Powder
  • Solubility
  • Water
  • Product Type
  • Regular
  • HS Code
  • 38210000
 

Antibiotic Assay Medium No 3 Trade Information

  • Minimum Order Quantity
  • 10 Pieces
  • Payment Terms
  • Cash Advance (CA), Cash in Advance (CID), Delivery Point (DP)
  • Supply Ability
  • 10 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Available
  • No
  • Packaging Details
  • Plastic Bottle
  • Main Export Market(s)
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • Main Domestic Market
  • All India
  • Certifications
  • Himedia
 

About Antibiotic Assay Medium No 3

Antibiotic Assay Medium No. 3, also known as Assay Broth, is a microbiological medium used to determine the potency of antibiotics in pharmaceutical and food products. It's specifically designed for the turbidimetric method, which measures the change or inhibition of growth of a test microorganism in a liquid medium containing varying concentrations of the antibiotic. Here's a more detailed description:Purpose:
  • Antibiotic Assay:This medium is primarily used for the microbiological assay of antibiotics, a process that determines the strength or effectiveness of an antibiotic.
  • Turbidimetric Method:The medium is specifically formulated for use in the turbidimetric method, where the growth of a test organism (usually bacteria) is measured by how much light it scatters (turbidity) in the liquid medium.
  • Quantitative Analysis:By measuring the turbidity, the potency of the antibiotic can be determined by comparing the results to a reference standard. 
Composition:
  • Peptone (Peptic Digest of Animal Tissue): Provides essential nutrients like nitrogen and amino acids for microbial growth.
  • Beef Extract: Another source of nitrogen and other growth factors.
  • Yeast Extract: A source of B-complex vitamins, crucial for microbial metabolism.
  • Dextrose (Glucose): Provides a readily available source of energy for microbial growth.
  • Sodium Chloride: Maintains osmotic equilibrium and ensures cell viability.
  • Dipotassium Phosphate and Monopotassium Phosphate: Act as buffering agents to maintain a stable pH. 
Function:
  1. 1. Nutrient Support:The medium provides the necessary nutrients for the growth of the test microorganism.
  2. 2. Growth Enhancement:Components like yeast extract and dextrose are included to promote robust and consistent growth of the test organism.
  3. 3. Buffering:The phosphate salts help to maintain a stable pH, which is crucial for the antibiotic's activity and the microorganism's growth.
  4. 4. Osmotic Balance:Sodium chloride maintains the appropriate salt concentration, preventing cell damage and ensuring accurate growth measurements. 
How it works in a turbidimetric assay:
  1. The antibiotic sample is diluted into different concentrations in the Assay Broth.
  2. The test microorganism is added to each dilution.
  3. The tubes are incubated under controlled conditions.
  4. After incubation, the turbidity (cloudiness) of each tube is measured using a spectrophotometer.
  5. The amount of growth (measured by turbidity) is compared to a standard curve of known antibiotic concentrations to determine the potency of the sample. 
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
Antibiotic Assay Medium No 3
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    AM SCIENTIFIC

    GST : 08EKWPP3090M1Z4

  • 2ND FLOOR, B-19, KANHA RESIDENCY, BHASKAR ENCLAVE 2, GOLYAWAS, MANSAROVAR,Jaipur - 302020, Rajasthan, India
  • Mr Mukesh Patel (Proprietor)
  • Mobile : 08045801108
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