Antibiotic Assay Medium No 36 500g

Antibiotic Assay Medium No. 36 is a specialized microbiological medium used for antibiotic assays, particularly for determining the potency of antibiotics. It is a general-purpose medium recommended for cultivating a wide range of microorganisms, including both fastidious and non-fastidious species. This medium is also used for sterility testing in pharmaceutical procedures and is referenced in the US Pharmacopeia. Key Features and Uses:

• Antibiotic Assays:It's a standard medium for antibiotic assays, especially for bleomycin, using Mycobacterium smegmatis as the test organism. 
• Microorganism Cultivation:It supports the growth of various microorganisms, making it suitable for a broad range of applications, including isolation and cultivation. 
• Sterility Testing:It is used for sterility testing in pharmaceutical settings, ensuring the absence of microbial contamination in products. 
• Seed Agar:It is often used as a seed agar for agar diffusion assays, a method for determining antibiotic potency. 
• Pharmaceutical Applications:It is widely used in pharmaceutical manufacturing for quality control and potency determination of antibiotics. 
• Maintenance of Cultures:It can be used to maintain stock cultures of microorganisms like Pseudomonas aeruginosa for antibiotic testing. 

Composition:While the exact formulation can vary slightly between manufacturers, it generally includes a peptone base, dextrose, and other components to support microbial growth. It may also be available in a vegetable-based (Veg) version, replacing animal-derived peptones with vegetable-based hydrolysates. Important Considerations:

• Freshly Prepared Plates:For accurate antibiotic assays, freshly prepared plates of Antibiotic Assay Medium No. 36 are recommended. 
• Sterilization:All materials and media must be properly sterilized before use to prevent contamination. 
• Storage:Store the dehydrated powder in a dry place, away from direct sunlight, and tightly sealed to prevent moisture absorption.