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Barritt Reagent A (for VP test) 100ml

Barritt Reagent A (for VP test) 100ml
Barritt Reagent A (for VP test) 100ml
Barritt Reagent A (for VP test) 100ml
Barritt Reagent A (for VP test) 100ml
Barritt Reagent A (for VP test) 100ml
Barritt Reagent A (for VP test) 100ml

Barritt Reagent A (for VP test) 100ml Specification

  • Packaging Size
  • 100 ml
  • Bottle Type
  • Plastic bottle
  • Test
  • Voges-Proskauer
  • State
  • Solution
  • Suitable For
  • Microbial testing
  • Pack Size
  • 100ml
  • GHS Status
  • Flammable
 

Barritt Reagent A (for VP test) 100ml Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • FOB Port
  • Jaipur
  • Payment Terms
  • Telegraphic Transfer (T/T), Cash in Advance (CID), Cash Advance (CA)
  • Supply Ability
  • 100 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Policy
  • Contact us for information regarding our sample policy
  • Main Export Market(s)
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Middle East, Western Europe, Africa
  • Main Domestic Market
  • All India
 

Barritt Reagent A (for VP test) 100ml About

HiMedia's Barritt Reagent A is a light yellow colored solution used with Barritt Reagent B in the Voges-Proskauer (VP) test to detect acetoin, a neutral end product of glucose fermentation by bacteria. A positive VP test, indicated by the formation of a cherry-red color after adding both Barritt reagents, helps identify bacteria like Enterobacter aerogenes. This reagent is a for in-vitro use, stored at 15-25C, and should not be exposed to skin or eyes.
 Purpose and Use
  • Voges-Proskauer Test: Barritt Reagent A is part of a two-reagent system used in the VP test. 
  • Acetoin Detection: The test identifies bacteria that ferment glucose to produce neutral, non-acidic end products, primarily acetoin (acetyl methyl carbinol). 
  • Bacterial Identification: The VP test is particularly useful for identifying members of the Enterobacteriaceae family. 
Procedure
  1. Inoculate a bacterial culture on MR-VP medium and incubate it for 24-48 hours. 
  2. Add Barritt Reagent A, followed by Barritt Reagent B (a potassium hydroxide solution). 
  3. Observe for the development of a cherry-red color within 5-10 minutes. 
Interpretation of Results 
  • Positive Test: A cherry-red color indicates the presence of acetoin and a positive VP test.
  • Negative Test: No red color formation signifies a negative VP test.
Storage and Safety
  • Storage: Store Barritt Reagent A at 15-25C. 
  • Safety: It is a combustible reagent and should be handled with care. Use appropriate personal protective equipment, such as gloves and eye protection, during handling. 
  • For Laboratory Use: This reagent is intended for in-vitro diagnostic and professional use only. 
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