Collagenase Type IV Solution

Collagenase Type IV Solution Specification

Ph Level
6-7

Shelf Life
12 Months

Storage
Room Temperature

Purity
99%

Solubility
Water Soluble

Usage
Laboratory

Grade
Industrial Grade

Container
Sterile vial

Endotoxin Level
<1 EU/mL

Transport
Shipped on ice

Pack Size
10 mL vial

Sterility
Sterile filtered

Buffer System
Hepes buffered saline

Concentration
200 U/mL

Collagenase Type IV Solution Trade Information

Minimum Order Quantity
10 Pieces

FOB Port
Jaipur

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)

Supply Ability
100 Pieces Per Day

Delivery Time
7 Days

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa

Main Domestic Market
All India

About Collagenase Type IV Solution

Packaging Size	100 ml
Brand	Himedia
Packaging Details	Plastic Bottle
Color	Orange
Country of Origin	Made in India
Appearance	Orange colored, clear solution
Hersteller	NeoFroxx
Make	Himedia
Pack Size	100Ml
Product Code	TCL117
Grade	LR (Laboratory)
Usage/Application	Laboratory
Type	Solution
Applikationen	IV

Minimum order quantity: 1 Piece

Collagenase Type IV is a specialized enzyme that breaks down type IV collagen, a key component of the basement membrane. It's derived from bacteria like Clostridium histolyticum. This enzyme is used in various applications, including isolating islet cells, dissociating tissues, and degrading extracellular matrix. Key Features and Applications:

Specificity:Collagenase Type IV has a specific affinity for the sequence R-Pro-(X-Gly-Pro), where X is often a neutral amino acid, allowing it to target type IV collagen.
Tissue Dissociation:It's effective in breaking down the intercellular matrix, which is crucial for separating cells from tissues.
Basement Membrane Degradation:It can degrade both type IV and type VII collagen, which are major components of the basement membrane.
Applications:
- Islet Isolation: Recommended for isolating Islets of Langerhans, which are important for insulin production.
- Cell Dissociation: Used in cell culture for isolating cells from tissues, especially when dealing with dense connective tissues.
- Stem Cell Research: Used in various stem cell research areas, including reprogramming, maintenance, and differentiation of both human and mouse pluripotent stem cells.
Storage and Preparation:
- Powdered Form: Stored at 2-8C.
- Solution: Reconstituted solution should be stored at -20C.
- Stock Solution: A 100mg/mL stock solution can be prepared by dissolving the powder in Hank's Balanced Salt Solution (HBSS).
- Filtering: The stock solution should be filter-sterilized before use.

Important Considerations:

Working Concentration:The optimal working concentration needs to be determined based on specific experimental conditions and the type of tissue being dissociated.
Secondary Proteases:Some collagenase preparations may contain clostripain and other secondary proteases, which can be a concern if they damage membrane proteins or receptors. Collagenase Type IV is designed to minimize these activities.
Safety:Collagenase is used in research and may not be suitable for direct human application without proper evaluation and approval.

Efficient Tissue Dissociation

Collagenase Type IV Solution delivers effective breakdown of extracellular matrix, making it ideal for studies involving cell isolation and tissue digestion. Its high activity and purity ensure reliable results while minimizing the risk of endotoxin interference, crucial when working with sensitive cell types.

Sterility and Convenient Handling

This product is sterile filtered and filled into a sterile vial, reducing the risk of contamination in your experiments. With water solubility and easy-to-use Hepes buffered saline, it integrates smoothly into standard laboratory workflows without additional preparation.

Stable and Flexible Storage

Unlike many enzymatic solutions, this Collagenase Type IV can be stored at room temperature for up to 12 months without compromising stability or activity. It is shipped on ice to preserve integrity during transit, ensuring researchers receive a product ready for immediate use.

FAQ's of Collagenase Type IV Solution:

Q: How should Collagenase Type IV Solution be stored upon arrival?

A: The solution is shipped on ice to preserve quality during transit, but it can be safely stored at room temperature for up to 12 months, ensuring convenient access without the need for refrigeration.

Q: What is the recommended application of this Collagenase Type IV Solution?

A: It is primarily intended for laboratory use, particularly for efficient tissue dissociation and cell isolation procedures in research and industrial settings.

Q: When is it advisable to use this specific grade of collagenase?

A: This industrial-grade collagenase is ideal for applications where high activity, purity (99%), and low endotoxin levels are necessary, such as preparing primary cell cultures from tissues.

Q: Where is this product manufactured and supplied from?

A: This Collagenase Type IV Solution is manufactured, distributed, exported, and supplied from India, providing reassurance of global reach and accessibility.

Q: What is the benefit of the Hepes buffered saline in the formulation?

A: The Hepes buffer maintains the solution's pH between 6 and 7, providing a stable environment for enzyme activity and supporting reproducible laboratory outcomes.

Q: How is sterility assured in this collagenase solution?

A: Sterility is achieved through filtration and packaging in sterile vials, helping to prevent microbial contamination in cell culture and tissue processing workflows.

Q: What are the process steps for using Collagenase Type IV Solution in laboratory protocols?

A: Simply dilute the sterile, water-soluble solution as required for your application, add to the tissue sample, and incubate to facilitate tissue dissociation; it integrates easily into standard laboratory protocols.

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