Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250 Specification

Packaging Type
Glass Bottle

Molecular Formula
C47H48N3NaO7S2

Usage
Laboratory

Physical State
Powder

Size
5g

CAS No
6104-58-1

Shelf Life
24 Months

Molecular Weight
854.04 GSM (gm/2)

Type
Scientific Lab Chemicals

Coomassie Brilliant Blue G 250 Trade Information

Minimum Order Quantity
10 Pieces

FOB Port
JAIPUR

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)

Supply Ability
100 Pieces Per Day

Delivery Time
5 Days

Sample Available
No

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Central America, North America, Eastern Europe, Africa, South America, Western Europe, Middle East, Asia, Australia

Main Domestic Market
All India

About Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G-250 (CBBG) is a dye used in analytical biochemistry, particularly for protein detection in electrophoresis and quantification via the Bradford assay. It's a triphenylmethane dye with a greenish tint, differentiated from Coomassie Brilliant Blue R-250 by the presence of two methyl groups. CBBG exists in different forms depending on pH, with the anionic (blue) form primarily binding to proteins. Key characteristics and uses:

Protein Detection:CBBG is widely used for visualizing proteins separated by SDS-PAGE and BN-PAGE.
Bradford Assay:It's a key component of the Bradford assay, a method for determining protein concentration.
pH Dependence:CBBG's color and binding affinity are pH-dependent. The anionic (blue) form, prevalent at pH above 2, is the form that binds to proteins, particularly those with basic or aromatic amino acid residues.
Forms:
- Cation (red form): Below pH 0, absorbance peak at 470 nm.
- Anion (blue form): Above pH 2, absorbance peak at 595 nm.
- Neutral (green form): Around pH 1, absorbance peak around 650 nm.
Solubility:CBBG is soluble in water, but methanol and acetic acid are often added for better solubility.
Destaining:In colloidal Coomassie blue staining, where CBBG is used in a colloidal dispersion, no destaining step is needed as the dye only binds to proteins and does not penetrate the gel matrix.
Alternatives:Other Coomassie dyes, like Coomassie Brilliant Blue R-250, are also used, but CBBG is often preferred for its higher sensitivity in the colloidal form.

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

Coomassie Brilliant Blue G 250

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