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Gordon McLeod Reagent Oxidase reagent 100ml

Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml
Gordon McLeod Reagent Oxidase reagent 100ml

Gordon McLeod Reagent Oxidase reagent 100ml Specification

  • Product Type
  • Regular
  • Physical Form
  • Liquid
  • Solubility
  • Water
  • CAS No
  • 2052-46-2
  • Usage
  • Laboratory
  • Storage
  • Room Temperature
  • Shelf Life
  • 12 Months
 

Gordon McLeod Reagent Oxidase reagent 100ml Trade Information

  • Minimum Order Quantity
  • 10 Pieces
  • Payment Terms
  • Days after Acceptance (DA), Cash Advance (CA), Cash in Advance (CID)
  • Supply Ability
  • 10 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Available
  • No
  • Packaging Details
  • Plastic Bottle
  • Main Export Market(s)
  • Australia, Asia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • Main Domestic Market
  • All India
  • Certifications
  • Himedia
 

About Gordon McLeod Reagent Oxidase reagent 100ml

The Gordon-McLeod reagent, also known as the oxidase reagent, is a solution used to detect the presence of cytochrome c oxidase enzyme in bacteria. This enzyme plays a crucial role in the electron transport chain and is a key factor in identifying certain bacteria, particularly pseudomonads and other oxidase-positive species, according to the American Society for Microbiology. Detailed Description:
  • Purpose:The reagent facilitates the oxidase test, a microbiological procedure used to differentiate bacteria based on their ability to produce cytochrome c oxidase. 
  • Composition:The reagent typically contains N,N,N,N-tetramethyl-p-phenylenediamine dihydrochloride (also known as tetramethyl-p-phenylenediamine) as the active component. This compound is a colorless reagent in its reduced form. 
  • Mechanism:When cytochrome c oxidase is present in a bacterial culture, it oxidizes the tetramethyl-p-phenylenediamine, causing it to change color from colorless to a dark blue or purple, indicating a positive result. 
  • Application:The oxidase test is used to help distinguish between different bacterial species, particularly Neisseria, Moraxella, Campylobacter, and Pasteurella, which are generally oxidase-positive, and other bacterial groups. 
  • Storage:Oxidase reagents are typically stored in a cool place, such as a refrigerator (2 to 8C) to maintain their stability and prevent degradation. 
  • Procedure:In the oxidase test, a small amount of the Gordon-McLeod reagent is applied to a filter paper or other suitable surface, and then bacterial colonies are smeared onto the paper. A positive result is indicated by the appearance of a blue or purple color within a short time (e.g., 10 seconds). 
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