HiCrome Chromogenic Coliform agar (CCA Agar) ,

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• Properties HiCrome Chromogenic Coliform Agar (CCA Agar) is a selective medium used to detect coliforms and Escherichia coli in water and food samples. It contains chromogenic substrates that detect the enzymes -galactosidase and -glucuronidase. What it detects: ColiformsProduce pink colonies due to the cleavage of one chromogen by the enzyme -galactosidase Escherichia coliProduce dark blue to violet colonies due to the cleavage of both chromogens by the enzymes -galactosidase and -glucuronidase How it works: The medium contains nutrients like peptone special and sodium pyruvate to help organisms grow Sodium lauryl sulfate inhibits gram-positive organisms L-Tryptophan improves the indole reaction, which increases detection reliability How to use: To confirm E. coli, add a drop of Kovacs reagent to the dark-blue to violet colony If the color turns cherry-red, the reaction is positive Storage: Store in a tightly closed container between 15-25 C Use before the expiry date on the label Dispose of used or unusable preparations by autoclaving or incineration
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