Kovacs Indole Reagent Himedia

Kovacs Indole Reagent Himedia Specification

Size
100ML

Color
Yellow

For Research Use
Yes

Container type
Glass bottle

Volume
100 ml

Sterility
Non-sterile

Ready to use
Yes

Kovacs Indole Reagent Himedia Trade Information

Minimum Order Quantity
1 Kilograms

FOB Port
jaipur

Payment Terms
Cash in Advance (CID), Cash Advance (CA), Paypal, Telegraphic Transfer (T/T)

Supply Ability
100 Kilograms Per Day

Delivery Time
3 Days

Sample Policy
Contact us for information regarding our sample policy

Main Domestic Market
All India

About Kovacs Indole Reagent Himedia

Packaging Size	100 ml
Grade	LR (Laboratory)
Brand	HiMedia
Physical State	Liquid
Usage / Application	Laboratory
Packaging Type	Glass Bottle
Color	Yellow
Country of Origin	India
Shelf Life	60 Months
Product Code	R008
Appearance	Yellow
pH Tpye	<1 (in HO at 20C)
Storage	2-8C (refrigerated)
Suitability	Detects indole production (tryptophanase activity) in bac
HSN Code	38229090

Minimum order quantity: 1 Kg

HiMedia's Kovac's Indole Reagent (R008) is a yellow liquid used in microbiology to detect if bacteria produce indole from tryptophan, indicated by a red ring forming at the top of the culture medium after adding the reagent, which contains p-dimethylaminobenzaldehyde, amyl alcohol, and hydrochloric acid, helping differentiate bacteria like E. coli (positive) from Enterobacter aerogenes (negative). Key Features & Use

Purpose: Detects indole production, a key step in differentiating bacteria, especially members of the Enterobacteriaceae family.
Mechanism: Microbes with tryptophanase break down tryptophan into indole; the reagent's p-dimethylaminobenzaldehyde reacts with indole to form a red color.
Composition: Typically contains p-dimethylaminobenzaldehyde, amyl alcohol (or isoamyl alcohol), and concentrated hydrochloric acid.
Positive Result: A red ring appears at the interface of the medium and the reagent layer.
Negative Result: No red ring forms.
Application: Used with tryptone broth cultures (e.g., 24-48 hours old) for microbial identification, not directly on clinical samples.
Storage: Keep tightly closed, in a dry, well-ventilated place, away from heat, and store refrigerated (2-8C) and protected from light to prevent degradation.

Precision in Indole Detection

Kovacs Indole Reagent offers a reliable and straightforward method for detecting indole production in various bacterial cultures. Its pre-mixed, ready-to-use format eliminates preparation errors, ensuring researchers can confidently achieve accurate results every time.

Made for Convenient Laboratory Use

Packaged in a 100 ml glass bottle, this yellow reagent ensures easy handling and storage. Its ready-to-use formula saves valuable laboratory time, while the non-sterile packaging keeps costs lower for standard research applications.

FAQ's of Kovacs Indole Reagent Himedia:

Q: How is Kovacs Indole Reagent used in microbiology research?

A: Kovacs Indole Reagent is used to detect indole production by certain bacteria, such as Escherichia coli. Simply add the reagent to a bacterial culture after incubation and observe for a red or pink ring, indicating a positive result.

Q: What is the process for performing the indole test using this reagent?

A: After growing the bacterial culture in the desired medium, add a few drops of Kovacs Indole Reagent to the test tube. A red or pink ring at the interface confirms indole production, while a yellow or no change suggests a negative result.

Q: When should researchers use this reagent in their workflow?

A: Researchers should use Kovacs Indole Reagent after incubating bacterial cultures to assess the capability of organisms to break down tryptophan and produce indole, an important step in bacterial identification.

Q: Where is Kovacs Indole Reagent by Himedia manufactured and distributed?

A: This reagent is manufactured, supplied, exported, and distributed by Himedia in India, serving laboratories and research institutions across the country and internationally.

Q: What are the main benefits of using the Kovacs Indole Reagent?

A: Key benefits include its ready-to-use formula, reliable detection of indole, and convenient 100 ml glass bottle packaging. It streamlines laboratory workflows and ensures consistent, accurate biochemical test results.

Q: Is Kovacs Indole Reagent suitable for all types of laboratory research?

A: Yes, Kovacs Indole Reagent is intended for research use in various settings, including educational, industrial, and clinical laboratories focused on microbiology and biochemical studies.

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