Lactophenol Cotton Blue

Lactophenol Cotton Blue Specification

Shelf Life
3 years

Boiling point
>100C

Storage Instructions
Store at room temperature, tightly closed, protected from light

Physical State
Liquid

Flash Point
>93C

CAS No
1324-76-1

Usage
For staining fungal elements and cell wall structures in microbiological studies

Melting Point
Not applicable (solution)

Size
100 mL, 500 mL, 1 L

Purity
>99%

Density
1.08 Gram per cubic centimeter(g/cm3)

Molecular Formula
C37H34N2Na2O9S3 (Cotton Blue component)

Packaging Type
HDPE Bottle or Glass Bottle

Molecular Weight
419.99 g/mol (Cotton Blue component)

Ph Level
Neutral

Grade
Laboratory Reagent

Type
Biological Stain

Application
Microscopy, Fungal and Bacterial Staining

Appearance
Blue solution

Purity(%)
>99%

Solubility
Completely miscible with water

Labeling Requirements
Must be labeled as hazardous reagent

Color Intensity
Deep Blue

Main Function
Differential staining of fungal cell walls

Composition
Phenol, Lactic Acid, Glycerol, Cotton Blue Dye, Water

pH Stability
Stable at pH 5-8

Odor
Phenolic

Format
Ready-to-use liquid reagent

Hazard Statement
Causes skin and eye irritation, handle with gloves and eye protection

Lactophenol Cotton Blue Trade Information

Minimum Order Quantity
1 Bottle

FOB Port
JAIPUR

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)

Supply Ability
100 Bottles Per Day

Delivery Time
7 Days

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa

Main Domestic Market
All India

About Lactophenol Cotton Blue

actophenol cotton blue (LPCB) is a staining solution used in microscopy to visualize fungi. It acts as both a stain and a mounting medium, with phenol acting as a disinfectant, lactic acid preserving the fungal structures, and cotton blue staining the fungal cell walls a blue color. This allows for the easy identification of fungal elements like spores and hyphae. Components and their functions

Phenol: Acts as a disinfectant, killing any living organisms in the sample.
Lactic acid: Preserves the structures of the fungi, preventing them from being distorted.
Cotton blue (Aniline blue): Stains the chitin in the fungal cell wall and other structures, giving them a blue color.
Glycerol: Prevents the sample from drying out under the microscope.

How it is used

A sample containing fungal material is placed on a glass slide.
A drop of lactophenol cotton blue stain is added to the sample.
A coverslip is placed over the slide.
The slide is then examined under a microscope, where the fungal structures will appear blue against a paler background.

Reliable Fungal Identification

Designed specifically for staining and visualizing fungal cell walls, Lactophenol Cotton Blue provides clear, intense contrast for easy microscopic examination. Its high purity and consistent color intensity help distinguish microscopic structures effectively, making it a top choice for both routine diagnostics and detailed research studies.

User-Friendly Application

Our reagent is supplied as a ready-to-use solution, simplifying the staining process for laboratory personnel. Its compatibility with standard microscopy techniques and stability at neutral pH ensure dependable results every time. The solution is safe to store at room temperature when protected from light and kept tightly sealed.

FAQ's of Lactophenol Cotton Blue:

Q: How should Lactophenol Cotton Blue be used for fungal staining?

A: Lactophenol Cotton Blue is applied directly to prepared microscope slides containing the specimen. Place a small drop of the reagent on the fungal sample and cover with a cover slip. The stain differentially colors cell wall structures, enabling clear visualization under a microscope.

Q: What safety precautions are necessary when handling this reagent?

A: Always wear gloves and eye protection when working with Lactophenol Cotton Blue, as it can cause skin and eye irritation. Handle it in a well-ventilated area and ensure the container is properly labeled as a hazardous reagent.

Q: When is it appropriate to use Lactophenol Cotton Blue in laboratory settings?

A: This stain is ideal for routine and specialized mycological studies, particularly when identifying and differentiating fungal elements in clinical, research, or teaching laboratories.

Q: Where should Lactophenol Cotton Blue be stored for best results?

A: Store the reagent at room temperature in tightly closed HDPE or glass bottles, protected from direct light. Proper storage maintains its efficacy throughout its three-year shelf life.

Q: What are the main benefits of using this stain in microscopy?

A: Lactophenol Cotton Blue provides deep blue, high-contrast staining of fungal cell walls, facilitating accurate identification and enhanced visualization of cellular structures under the microscope.

Q: Is the solution completely miscible with water, and how stable is it?

A: Yes, Lactophenol Cotton Blue is completely miscible with water and remains stable within a pH range of 5-8, ensuring reliable performance in laboratory use.

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