Merck ReadyCult

About Merck ReadyCult

Packaging Size	20 blisters
Media Type	Selective enrichment & indicator medium
Grade	Microbiological / Laboratory Grade
Application	Laboratory
Pack Size	20 blisters
Usage / Application	Laboratory
Country of Origin	India
Form	Ready-to-use blister
Appearance	Light-colored, compact granules that dissolve when added to 100 mL water sample to form
Brand	Merck

Minimum order quantity: 1 Kg

Readycult Coliforms 100 by Merck (Sigma-Aldrich) is a rapid, ready-to-use chromogenic culture medium in a snap-pack for detecting total coliforms and E. coli in 100 mL water samples, turning blue-green for coliforms and showing blue fluorescence under UV for E. coli, offering an easy, quick, EPA-approved test for drinking water quality.} Key Features:

• Simultaneous Detection: Detects total coliforms and E. coli in one step.
• Chromogenic/Fluorogenic: Uses X-GAL (cleaved by coliforms for blue-green color) and MUG (cleaved by E. coli for blue fluorescence under UV).
• Easy-to-Read: Provides clear, definitive color change (yellow to blue-green) for coliforms and fluorescence for E. coli.
• Rapid & Convenient: Supplied in single-use snap-packs for fast preparation, giving results within 18-24 hours.
• Selective: Lauryl sulfate inhibits most other bacteria, especially Gram-positives.
• EPA Approved: Acceptable for monitoring drinking water under the Total Coliform Rule. 

How it Works:

1. Add to Sample: A single snap-pack is added to 100 mL of water.
2. Incubation: Incubate for 18-24 hours.
3. Positive for Coliforms: The medium turns blue-green if total coliforms are present.
4. Positive for E. coli: If E. coli is present, it causes blue fluorescence under a UV light (365 nm), in addition to the blue-green color.
5. Optional Confirmation: An indole test can be performed for definitive E. coli confirmation. 

 

Reliable Total Coliform Detection

Merck ReadyCult offers swift and accurate identification of total coliforms in water samples using a straightforward color change. After just 18-24 hours of incubation, users can visually confirm coliform presence by observing a shift to yellow, streamlining the water quality monitoring process.


Convenient and Sterile to Use

This product requires no additional preparation-simply mix the sterile white powder into water. Packed in 100 mL bottles, ReadyCult ensures convenient handling, reliable sterility, and reduced risk of contamination throughout testing.


Long-Lasting Performance and Easy Storage

ReadyCult maintains its integrity and effectiveness for up to 12 months when stored at room temperature (2-25C). Its stable formulation and water solubility make it an excellent choice for facilities needing consistent and accurate results over extended periods.