MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia

MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia Specification

Shelf Life
12 Months

Grade
Industrial Grade

CAS No
143-74-8

Ph Level
6-90-7.30

Physical Form
Liquid

Purity
99%

Storage
Room Temperature

Suitability
ISO 7932 standard

Egg yolk reaction
Lecithinase detection

Sterilization
Autoclave 121°C, 15 min

Inhibitor
Polymyxin B

Colony appearance
Pink with egg yolk precipitation

Target organism
Bacillus cereus

Solidification agent
Agar

Recommended volume
25 g/L medium

Colour change indicator
Phenol red

MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia Trade Information

Minimum Order Quantity
10 Pieces

FOB Port
Jaipur

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)

Supply Ability
100 Pieces Per Day

Delivery Time
7 Days

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa

Main Domestic Market
All India

About MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia

Packaging Size	500 g
Packaging Details	Plastic Bottle
Usage/Application	Laboratory
Country of Origin	Made in India
CAS NO	143-74-8
Pack Size	500g
PH	6-90-7.30
Product Code	M636
Brand	Himedia

Minimum order quantity: 1 Gram

Himedia's MYP Agar Base is used for the selective isolation and identification of Bacillus cereus and pathogenic Staphylococci. The medium uses Phenol Red to indicate mannitol fermentation (yellow colonies), egg yolk to reveal lecithinase activity (white precipitate zones), and Polymyxin B Sulfate to inhibit Gram-negative bacteria. Purpose

Isolate and identify pathogenic Staphylococci and Bacillus species.
Differentiate Bacillus cereus from other Bacillus species.

Components and their functions

Peptone and Meat Extract:Provide nitrogen, carbon, vitamins, and minerals for bacterial growth.
D-Mannitol:A carbohydrate source that, when fermented by bacteria, produces acid and lowers the pH.
Phenol Red:A pH indicator that turns colonies yellow when mannitol is fermented, indicating a mannitol-fermenting organism.
Egg Yolk Emulsion:A differential component that produces a white precipitate zone around lecithinase-producing colonies, such as Staphylococcus aureus and some Bacillus species.
Polymyxin B Sulfate:A selective agent that restricts the growth of Gram-negative bacteria, like E. coli and Pseudomonas aeruginosa.
Agar:The solidifying agent that provides the gel-like consistency for the medium.

How to interpret results

Yellow colonies with a white precipitate zone:Indicates a mannitol-fermenting, lecithinase-producing organism (e.g., Staphylococcus aureus).
Yellow colonies without a white precipitate zone:Suggests a mannitol-fermenting, lecithinase-negative organism (e.g., Bacillus subtilis).
No yellow color with a white precipitate zone:Indicates a non-mannitol-fermenting, lecithinase-positive organism (e.g., Bacillus cereus).

Robust Detection of Bacillus cereus

The MYP Agar Base utilizes phenol red for clear colour change and egg yolk for effective lecithinase activity detection. This allows accurate identification and differentiation of Bacillus cereus by its characteristic pink colonies with egg yolk precipitation, making the process reliable for food safety and quality control.

Compliant with International Standards

Developed according to ISO 7932 requirements, this agar base ensures compatibility with global methods for detecting Bacillus cereus. Its consistent and reproducible results make it the preferred choice for routine industrial and laboratory applications, providing confidence in microbiological testing.

Simple Preparation and Storage

Supplied in a liquid, industrial-grade format with 99% purity, the medium is easy to prepare: simply add 25 g per litre and sterilize by autoclaving at 121C for 15 minutes. The long shelf life of 12 months and requirement for room temperature storage promote convenience and cost-effectiveness.

FAQ's of MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base) Himedia:

Q: How is MYP Agar Base Phenol Red Egg Yolk Polymyxin Agar Base prepared for use?

A: Dissolve 25 g of the medium per litre of distilled water, mix thoroughly, and sterilize by autoclaving at 121C for 15 minutes before pouring into Petri dishes for laboratory or industrial application.

Q: What is the primary application of this agar base?

A: It is primarily used for the isolation and enumeration of Bacillus cereus, particularly in food, environmental, and industrial microbiology according to ISO 7932 standard.

Q: When will I observe colour changes or egg yolk reactions?

A: Colour change to pink and the appearance of egg yolk precipitation-indicative of lecithinase production-become visible after sufficient incubation of Bacillus cereus on the medium.

Q: Where should I store the MYP Agar Base to ensure maximum shelf life?

A: Store the agar base at room temperature as recommended, which helps maintain its 12-month shelf life and preserves its effectiveness and purity.

Q: What is the benefit of using phenol red and polymyxin B in this medium?

A: Phenol red enables rapid pH-based colour indication of bacterial activity, while polymyxin B suppresses non-target organisms, ensuring selective growth of Bacillus cereus for more accurate results.

Q: How does the medium facilitate lecithinase detection?

A: The inclusion of egg yolk allows the visualization of lecithinase activity produced by Bacillus cereus as an opaque, precipitated zone around colonies.

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