Sabouraud Dextrose Agar, Granulated Himedia

Sabouraud Dextrose Agar, Granulated Himedia Specification

Purity
99%

Usage
Laboratory

Shelf Life
12 Months

Grade
Analytical Grade

Physical Form
Granule

Storage
Room Temperature

Solubility
soluble in water and insoluble

Ph Level
5.40 - 5.80

Recommended Sterilization
121°C for 15 min

Typical Fill Weight
500g pack

pH After Sterilization
5.6 ±0.2

Moisture Content
Not more than 10%

Medium Code
M063

Sabouraud Dextrose Agar, Granulated Himedia Trade Information

Minimum Order Quantity
10 Pieces

FOB Port
Jaipur

Payment Terms
Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)

Supply Ability
100 Pieces Per Day

Delivery Time
7 Days

Sample Policy
Contact us for information regarding our sample policy

Main Export Market(s)
Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa

Main Domestic Market
All India

About Sabouraud Dextrose Agar, Granulated Himedia

Packaging Size	500 g
Packaging Details	Plastic Bottle
Usage/Application	Laboratory
Country of Origin	Made in India
Pack Size	500g
Physical Form	Granular
Recommended Storage Temperature	On receipt store between 10-30C
Specific end uses	Apart from the uses mentioned in section 1.2 no other specific uses are stipulated
Packaging type	HDPE
Appearance	Cream to yellow coloured granular medium
pH	5.40 - 5.80
Grade	AR (Analytical)
Brand	Himedia
Product Type	Granulated

Minimum order quantity: 1 Kg

HiMedia's Sabouraud Dextrose Agar, Granulated is a microbiology medium used for isolating and cultivating fungi like yeasts and molds, especially those associated with skin infections. Its low pH (around 5.6) and high dextrose concentration inhibit the growth of most bacteria, making it selective for fungi. The granulated form offers ease of handling, dissolution, and storage compared to traditional powdered media. Key features

Selective growth: The medium's acidic pH and high sugar content are specifically designed to suppress bacterial growth, allowing fungi like yeasts, molds, and dermatophytes to thrive.
Wide applications: It is used in both clinical settings and for general microbiology to cultivate fungi from various specimens, including those from skin, and can also be used to test antimycotic substances.
Granulated form: The granulated format ensures quick and complete dissolution, better stability, and is easy to handle and weigh.
Supports aciduric organisms: Besides fungi, it also supports the growth of acid-tolerant (aciduric) microorganisms.
Inhibits bacteria: The low pH (around 5.6) is a key feature that prevents the growth of most bacteria in the sample.

How to use

For isolation from contaminated samples, consider using a selective medium simultaneously with Sabouraud Dextrose Agar.
Incubate cultures for an extended period (up to 4-6 weeks) before reporting them as negative for fungal growth, especially for dermatophytes.

Reliable Medium for Fungal Cultivation

Sabouraud Dextrose Agar from Himedia is specifically formulated for the effective isolation of fungi, including dermatophytes and other pathogenic species. Its granulated form ensures precise measurement and consistent performance, making it an essential tool for laboratories engaged in mycological studies or clinical diagnostics. With analytical grade standards, users can trust the reliability of results generated using this medium.

Easy Preparation and Sterilization

This medium boasts excellent water solubility and is simple to prepare. For optimal performance, sterilize at 121C for 15 minutes before use. The granulated formulation allows for uniform mixing, resulting in a consistent surface for fungal growth. Its recommended pH and fill weight further support its suitability for high-quality laboratory applications.

FAQ's of Sabouraud Dextrose Agar, Granulated Himedia:

Q: How do I prepare Sabouraud Dextrose Agar, Granulated Himedia for use in the laboratory?

A: To prepare the medium, dissolve the required amount of granulate in distilled water, sterilize at 121C for 15 minutes, and allow it to cool before pouring into petri plates or tubes. Ensure the pH after sterilization is maintained between 5.6 0.2 for optimal fungal growth.

Q: What is the primary application of Sabouraud Dextrose Agar in research and diagnostics?

A: Sabouraud Dextrose Agar is mainly used for the isolation and cultivation of fungi, especially yeasts and molds, in clinical, pharmaceutical, and food industry laboratories. Its formulation supports rapid and effective fungal growth assessment.

Q: When should the medium be discarded or replaced after opening?

A: The medium has a shelf life of 12 months when stored at room temperature with the packaging intact. If signs of contamination or altered physical characteristics are observed after opening, the product should be replaced immediately.

Q: Where is Sabouraud Dextrose Agar typically stored?

A: It should be stored at room temperature, away from moisture and direct sunlight, in a tightly sealed container to preserve its integrity and prevent premature degradation.

Q: What sterilization process is recommended for this agar prior to use?

A: Sterilization should be conducted in an autoclave at 121C for 15 minutes as per standard laboratory protocols, ensuring the medium's suitability for sensitive fungal cultures.

Q: Can you explain the benefit of using the analytical grade, granulated form of this medium?

A: The analytical grade and granulated form ensure high purity (99%), ease of handling, precise measurement, and thorough dissolution in water. This facilitates reproducible results and consistent performance in laboratory applications.

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