Safranine Staining Powder Qualigens

About Safranine Staining Powder Qualigens

Qualigens Safranine Staining Powder is a high-quality biological stain, a red cationic dye used for microscopy and staining, most commonly as a counterstain in the Gram stain procedure to color Gram-negative bacteria pink to red. It also stains bacterial spores and plant nuclei, and is useful for demonstrating glycosaminoglycans (GAGs) in cartilage by imparting an intense red color to them. The powder is a solid, brownish-red to dark brown substance, soluble in water and ethanol, and is typically a key reagent in histology, cytology, and botanical studies for visualizing tissue and cellular structures. Key characteristics and uses:

• Chemical Nature: Safranin O (also known as Basic Red 2) is a cationic (positively charged) dye. 
• Color: It imparts an intense red color, making it a visible stain for various biological components. 
• Staining Applications:
  • Gram Staining: Used as a counterstain to identify Gram-negative bacteria, which appear pink to red. 
  • Plant Anatomy: Stains plant nuclei and is used in conjunction with other stains like Safranine-fast green FCF for plant cells. 
  • Cartilage and GAGs: Specifically binds to polyanions like proteoglycans (a type of GAG) in cartilage, staining them intensely red and helping to assess their depletion. 
  • Bacterial Spores: Can be used for staining bacterial spores. 
• Physical Form: It is supplied as a solid powder, with a brownish-red to dark brown appearance. 
• Solubility: The powder is soluble in water and ethanol, allowing for the preparation of staining solutions. 
• Quality: Qualigens Safranine powder is a premium reagent designed for precise and reliable staining in biological research and laboratory applications.

Key Features and Composition

This Safranine Staining Powder Qualigens is notable for its 99% purity, water solubility, and AR-grade quality. It is specifically formulated for laboratory staining purposes where high-quality and consistent results are required. The powder is supplied in 25 g bottles, making it convenient for both small-scale and large-scale laboratory use.


Staining Applications and Benefits

Widely used as a basic stain in analytical processes, Safranine is invaluable for microscopic tissue studies, bacterial staining, and botanical research. Its high pH accuracy (6-7) and outstanding purity ensure that laboratory outcomes are both reliable and reproducible, while its non-flammable classification enhances lab safety.


Safe Handling and Storage Guidelines

This product should be stored at room temperature to maintain optimal shelf life (up to 12 months). As a non-flammable substance with water solubility, it poses minimal risk during storage and handling. Always follow standard laboratory protocols when preparing and using the dye to ensure safety and effectiveness.