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Violet Red Bile Agar Himedia 100g

Violet Red Bile Agar Himedia 100g
Violet Red Bile Agar Himedia 100g
Violet Red Bile Agar Himedia 100g
Violet Red Bile Agar Himedia 100g
Violet Red Bile Agar Himedia 100g
Violet Red Bile Agar Himedia 100g

Violet Red Bile Agar Himedia 100g Specification

  • Molecular Formula
  • N/A mixture
  • Purity
  • N/A formulated medium
  • Density
  • Not specified Gram per cubic centimeter(g/cm3)
  • Molecular Weight
  • N/A mixture Grams (g)
  • Shelf Life
  • 4 Years
  • Size
  • 100g
  • Physical State
  • Powder
  • Usage
  • Laboratory
  • Packaging Type
  • Plastic Bottle
  • Boiling point
  • N/A
  • CAS No
  • N/A mixture
  • Ph Level
  • 7.4 0.2
  • Melting Point
  • N/A
  • Flash Point
  • N/A
  • Grade
  • Microbiology Grade
  • Type
  • Scientific Lab Chemicals
  • Application
  • Laboratory
  • Appearance
  • Light yellow to pink powder
  • Purity(%)
  • N/A formulated medium
 

Violet Red Bile Agar Himedia 100g Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • Payment Terms
  • Cash Advance (CA), Cash in Advance (CID), Days after Acceptance (DA)
  • Supply Ability
  • 10 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Available
  • No
  • Packaging Details
  • Plastic Bottle
  • Main Export Market(s)
  • North America, South America, Eastern Europe, Western Europe, Asia, Australia, Central America, Middle East, Africa
  • Main Domestic Market
  • All India
 

About Violet Red Bile Agar Himedia 100g

Violet Red Bile Agar (VRBA) is a selective culture medium used to isolate, detect, and enumerate coliform bacteria, particularly in water, milk, dairy products, and clinical samples. It achieves this by inhibiting the growth of Gram-positive bacteria while allowing lactose-fermenting coliforms to grow and produce characteristic red colonies. Here's a more detailed breakdown:Composition and Principle:
  • Selective Agents:VRBA contains bile salts and crystal violet, which inhibit the growth of Gram-positive bacteria and some Gram-negative bacteria.
  • Indicator:Neutral red acts as a pH indicator. When lactose is fermented by coliforms, acid is produced, causing the neutral red to turn red, resulting in red or pink colonies.
  • Lactose:This is the fermentable sugar that coliforms utilize, producing acid and leading to the color change.
  • Agar:This provides a solidifying agent for the medium. 
Applications:
  • Water and Dairy Products:VRBA is widely used in the analysis of water, milk, and other dairy products to detect and quantify coliform bacteria, which are indicators of potential fecal contamination. 
  • Clinical Samples:It can also be used in clinical settings to isolate and identify coliforms from various samples. 
  • Food Safety:It's also used in food microbiology to assess the quality and safety of food products. 
Increasing Selectivity:
  • Anaerobic Incubation: Incubating VRBA under anaerobic conditions can further suppress the growth of facultative anaerobes, enhancing the selectivity for coliforms.
  • Elevated Temperatures: Incubation at temperatures above 42C can also improve selectivity. 
Interpretation:
  • Lactose Fermenters:Coliforms that ferment lactose produce red or pink colonies, often surrounded by a reddish zone due to bile precipitation. 
  • Non-Lactose Fermenters:Bacteria that do not ferment lactose will produce colorless or pale colonies. 
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