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Antibiotic Assay Medium No 19

Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19
Antibiotic Assay Medium No 19

Antibiotic Assay Medium No 19 Specification

  • Solubility
  • Water
  • Shelf Life
  • 12 Months
  • Product Type
  • Regular
  • Physical Form
  • Powder
  • Usage
  • Laboratory
  • Storage
  • Room Temperature
  • HS Code
  • 38210000
 

Antibiotic Assay Medium No 19 Trade Information

  • Minimum Order Quantity
  • 10 Pieces
  • Payment Terms
  • Cash Advance (CA), Cash in Advance (CID), Days after Acceptance (DA)
  • Supply Ability
  • 10 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Available
  • No
  • Packaging Details
  • Plastic Bottle
  • Main Export Market(s)
  • Asia, Australia, Central America, North America, South America, Eastern Europe, Western Europe, Middle East, Africa
  • Main Domestic Market
  • All India
  • Certifications
  • Himedia
 

About Antibiotic Assay Medium No 19

Antibiotic Assay Medium No. 19 is a microbiological medium used for the bioassay of various antibiotics, particularly Amphotericin B, Nystatin, and Candicidin, using Saccharomyces cerevisiae as the indicator organism. It is a nutrient-rich medium providing essential elements for microbial growth and is used in both the base and seed agar for diffusion assays. Here's a more detailed description:Composition:
  • Peptone (9.4 g/L): Provides a source of nitrogen, vitamins, and amino acids.
  • Yeast Extract (4.7 g/L): Supplies vitamins, amino acids, and other growth factors.
  • Beef Extract (2.4 g/L): Contributes to the nutrient base with nitrogen and other essential components.
  • Dextrose (10.0 g/L): Acts as a carbon and energy source for microbial growth.
  • Sodium Chloride (10.0 g/L): Maintains the osmotic balance of the medium, crucial for cell integrity.
  • Agar (23.5 g/L): A solidifying agent that forms a gel base for the assay. 
Intended Use:
  • Antibiotic Assays:Specifically designed for the microbiological assay of antibiotics like Amphotericin B, Nystatin, and Candicidin, which are antifungal agents. 
  • Cylinder Plate Method:Commonly used with the cylinder plate method (also known as the cup-plate method) where antibiotic solutions diffuse from cylinders into the agar, creating zones of inhibition that are measured to determine antibiotic potency. 
  • Seed and Base Agar:Employed as both the base and seed medium in agar diffusion assays. 
  • USP/EP Compliance:Formulated according to the specifications of the United States Pharmacopeia (USP) and the European Pharmacopoeia (EP). 
Key Features:
  • Nutrient-rich:Provides a wide range of nutrients for the growth of Saccharomyces cerevisiae. 
  • Osmotic Balance:Sodium chloride maintains the correct osmotic pressure for cell stability. 
  • Standardized:The formula is adjusted and standardized to ensure consistent and reliable results in antibiotic assays. 
  • BSE/TSE Free:In some formulations, such as Antibiotic Veg Assay Medium No. 19, vegetable peptones replace animal peptones, eliminating risks associated with bovine spongiform encephalopathy (BSE) and transmissible spongiform encephalopathy (TSE). 
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