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Antibiotic Assay Medium No 2 Base Agar 500g Himedia

Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia

Antibiotic Assay Medium No 2 Base Agar 500g Himedia Specification

  • Melting Point
  • N/A
  • Purity
  • N/A complex microbiological medium
  • Packaging Type
  • Plastic Bottle
  • Usage
  • Laboratory
  • CAS No
  • N/A complex mixture
  • Molecular Weight
  • N/A complex mixture Carat
  • Flash Point
  • N/A / not determined
  • Ph Level
  • 6.6 0.2
  • Physical State
  • Solid
  • Density
  • Not specified by manufacturer Gram per cubic centimeter(g/cm3)
  • Molecular Formula
  • N/A complex mixture
  • Boiling point
  • N/A
  • Size
  • 500g
  • Shelf Life
  • 3 Years
  • Grade
  • Microbiological / bacteriological culture-media grade
  • Type
  • Other
  • Application
  • Laboratory
  • Appearance
  • Cream to yellow, homogeneous, free-flowing powder
  • Purity(%)
  • N/A complex microbiological medium
  • Product Code
  • M005
  • Color
  • Amber coloured
 

Antibiotic Assay Medium No 2 Base Agar 500g Himedia Trade Information

  • Minimum Order Quantity
  • 10 Pieces
  • Supply Ability
  • 100 Pieces Per Month
  • Delivery Time
  • 7 Days
 

About Antibiotic Assay Medium No 2 Base Agar 500g Himedia

Antibiotic Assay Medium No. 2 Base Agar is a solid, solidified medium used as a base layer in microbiological assays for determining antibiotic potency. It provides a stable surface for the diffusion of the antibiotic into a seed agar layer inoculated with test organisms. The medium's composition, typically including peptone, yeast extract, and beef extract, provides the necessary nutrients for the growth of the test organisms while also supporting the over-layering of the seed agar. Key Features and Usage:
  • Base Agar:This medium serves as the foundation for antibiotic assays, particularly those involving agar diffusion techniques like the cylinder-plate method.
  • Nutrient-Rich:Peptone, yeast extract, and other ingredients provide essential nutrients (carbon, nitrogen, vitamins) for the growth of test organisms, allowing for clear zone measurements.
  • Solidified Substrate:The agar component provides the solid, stable base layer needed for the diffusion of the antibiotic and the growth of the test organisms in the seed agar layer.
  • Standardized:The medium is designed to meet specific requirements for microbiological assays, as outlined in pharmacopoeias and standard testing methods. 
Preparation and Use:
  1. 1. Preparation:The medium is typically prepared by dissolving the dehydrated powder in distilled or deionized water, heating to boiling, and sterilizing by autoclaving.
  2. 2. Base Layer:Once sterilized, the medium is poured into Petri dishes to create a solid base layer.
  3. 3. Seed Agar:A seed agar layer, inoculated with the test organism, is then carefully layered over the base agar.
  4. 4. Diffusion:The antibiotic, in the form of a disc, cylinder, or well, is placed on the base agar, allowing the antibiotic to diffuse into the seeded agar.
  5. 5. Zone Measurement:As the antibiotic diffuses, it inhibits the growth of the test organism, creating a clear zone around the antibiotic source. The size of this zone is then measured to determine the antibiotic's potency or activity. 
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
Antibiotic Assay Medium No 2 Base Agar 500g Himedia
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