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Cetrimide Agar 500g Himedia

Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia

Cetrimide Agar 500g Himedia Specification

  • Shelf Life
  • 3 Years
  • Physical State
  • Solid
  • Molecular Formula
  • Not applicable
  • Flash Point
  • Not applicable
  • Density
  • Not specified Gram per cubic centimeter(g/cm3)
  • Storage Instructions
  • 15-25 C
  • Size
  • 500g
  • Molecular Weight
  • Not applicable Carat
  • Usage
  • Laboratory
  • Melting Point
  • Not applicable
  • Purity
  • Not applicable
  • Ph Level
  • 7.2 0.2
  • Packaging Type
  • Plastic Bottle
  • Boiling point
  • Not applicable
  • CAS No
  • 8044-71-1
  • Grade
  • Microbiology / bacteriological culture media grade
  • Type
  • Other
  • Application
  • Laboratory
  • Appearance
  • Cream to yellow, homogeneous free-flowing powder
  • Purity(%)
  • Not applicable
  • Product Code
  • MH024
  • Formulation
  • Dehydrated medium
 

Cetrimide Agar 500g Himedia Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • Supply Ability
  • 100 Pieces Per Month
  • Delivery Time
  • 7 Days
 

About Cetrimide Agar 500g Himedia

Cetrimide agar is a selective and differential medium used to isolate and identify Pseudomonas aeruginosa from various samples. It contains cetrimide, a detergent that inhibits the growth of most bacteria except for P. aeruginosa and some other Pseudomonas species. The medium also enhances the production of pyocyanin, a blue-green pigment, and pyoverdin, a yellow-green fluorescent pigment, which are characteristic of P. aeruginosa. Key Features and Applications:
  • Selective Medium:Cetrimide agar is selective because cetrimide inhibits the growth of many other bacteria, allowing P. aeruginosa to grow preferentially. 
  • Differential Medium:The medium can differentiate P. aeruginosa from other Pseudomonas species based on its ability to produce pyocyanin and pyoverdin. 
  • Isolation and Identification:It's used for the isolation of P. aeruginosa from various samples, including clinical specimens, environmental samples, and pharmaceutical products. 
  • Harmonized Method:The medium is used in microbial limit testing according to harmonized methodologies of USP/EP/BP/JP/IP. 
  • Pigment Production:P. aeruginosa grown on cetrimide agar produces pyocyanin (a blue, water-soluble pigment) and pyoverdin (a yellow-green fluorescent pigment). 
Composition (Typical):
  • Pancreatic Digest of Gelatin: A source of nitrogen.
  • Magnesium Chloride: Enhances pyocyanin production and neutralizes EDTA.
  • Dipotassium Sulfate: Enhances pyocyanin production.
  • Cetrimide: The selective agent that inhibits the growth of most bacteria.
  • Agar: The solidifying agent.
  • Glycerol: A source of carbon. 
Preparation and Usage:
  1. 1. Dehydrated Form:Cetrimide agar is available in a dehydrated form, which is dissolved in distilled water and autoclaved. 
  2. 2. Pouring Plates:The medium is cooled and poured into sterile Petri dishes. 
  3. 3. Inoculation:Samples are inoculated onto the agar plates and incubated at appropriate temperatures (typically 37C for clinical samples and 30-35C for some other Pseudomonas species). 
  4. 4. Identification:P. aeruginosa colonies are typically identified by their characteristic blue-green pigment production and, in some cases, a grape-like odor. 
Important Notes:
  • P. aeruginosa is a common nosocomial pathogen and can cause various infections, including pneumonia, bacteremia, and urinary tract infections. 
  • The characteristic blue-green pigment (pyocyanin) and fluorescent pigment (pyoverdin) production help differentiate P. aeruginosa from other Pseudomonas species and other bacteria. 
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