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Cetrimide Agar 500g Himedia

Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia

Cetrimide Agar 500g Himedia Specification

  • Shelf Life
  • 3 Years
  • Ph Level
  • 7.2 0.2
  • Molecular Weight
  • N/A complex mixture Carat
  • Boiling point
  • N/A
  • Size
  • 500g
  • Melting Point
  • N/A
  • Physical State
  • Solid
  • Flash Point
  • N/A / not determined
  • Packaging Type
  • Plastic Bottle
  • Purity
  • Not applicable
  • Usage
  • Laboratory
  • Storage Instructions
  • Store Between 10-30 DegreeC
  • Molecular Formula
  • N/A complex mixture
  • Density
  • Not specified Gram per cubic centimeter(g/cm3)
  • CAS No
  • N/A complex mixture
  • Grade
  • Microbiological / bacteriological culture-media grade
  • Type
  • Other
  • Application
  • Laboratory
  • Appearance
  • Cream to yellow, homogeneous, free-flowing powder
  • Purity(%)
  • Not applicable
  • Prepared Medium
  • Light Amber, Opalescent Gel
  • Form
  • Powder
  • Color
  • Yellow
  • Gelling
  • Firm, 1.36% Agar
  • Cultural Response
  • Observed 30-35C, 18-72h
  • Product Code
  • MH024
 

Cetrimide Agar 500g Himedia Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • Supply Ability
  • 100 Pieces Per Month
  • Delivery Time
  • 5 Days
 

About Cetrimide Agar 500g Himedia

Cetrimide agar is a selective and differential medium used to isolate and identify Pseudomonas aeruginosa from various samples. It contains cetrimide, a detergent that inhibits the growth of most bacteria except for P. aeruginosa and some other Pseudomonas species. The medium also enhances the production of pyocyanin, a blue-green pigment, and pyoverdin, a yellow-green fluorescent pigment, which are characteristic of P. aeruginosa. Key Features and Applications:
  • Selective Medium:Cetrimide agar is selective because cetrimide inhibits the growth of many other bacteria, allowing P. aeruginosa to grow preferentially. 
  • Differential Medium:The medium can differentiate P. aeruginosa from other Pseudomonas species based on its ability to produce pyocyanin and pyoverdin. 
  • Isolation and Identification:It's used for the isolation of P. aeruginosa from various samples, including clinical specimens, environmental samples, and pharmaceutical products. 
  • Harmonized Method:The medium is used in microbial limit testing according to harmonized methodologies of USP/EP/BP/JP/IP. 
  • Pigment Production:P. aeruginosa grown on cetrimide agar produces pyocyanin (a blue, water-soluble pigment) and pyoverdin (a yellow-green fluorescent pigment). 
Composition (Typical):
  • Pancreatic Digest of Gelatin: A source of nitrogen.
  • Magnesium Chloride: Enhances pyocyanin production and neutralizes EDTA.
  • Dipotassium Sulfate: Enhances pyocyanin production.
  • Cetrimide: The selective agent that inhibits the growth of most bacteria.
  • Agar: The solidifying agent.
  • Glycerol: A source of carbon. 
Preparation and Usage:
  1. 1. Dehydrated Form:Cetrimide agar is available in a dehydrated form, which is dissolved in distilled water and autoclaved. 
  2. 2. Pouring Plates:The medium is cooled and poured into sterile Petri dishes. 
  3. 3. Inoculation:Samples are inoculated onto the agar plates and incubated at appropriate temperatures (typically 37C for clinical samples and 30-35C for some other Pseudomonas species). 
  4. 4. Identification:P. aeruginosa colonies are typically identified by their characteristic blue-green pigment production and, in some cases, a grape-like odor. 
Important Notes:
  • P. aeruginosa is a common nosocomial pathogen and can cause various infections, including pneumonia, bacteremia, and urinary tract infections. 
  • The characteristic blue-green pigment (pyocyanin) and fluorescent pigment (pyoverdin) production help differentiate P. aeruginosa from other Pseudomonas species and other bacteria. 
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
Cetrimide Agar 500g Himedia
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