CLED Agar Bromo Thymol Blue 500g Himedia

C.L.E.D. Agar w/Bromo Thymol Blue is recommended for use in urinary bacteriology. This medium promotes the growth of all urinary pathogens. Lactose is the fermentable sugar. Bromo thymol blue is the pH indicator which turns yellow at acidic pH. Colonies grow as blue or yellow due to fermentation of lactose. HiMedia M792 is C.L.E.D. (Cystine Lactose Electrolyte Deficient) Agar with Bromo Thymol Blue, a microbiological medium used to isolate, count, and differentiate urinary pathogens by detecting lactose fermentation (yellow colonies for fermenters, blue/purple for non-fermenters). Its key specs include a composition of peptones, tryptone, lactose (10g/L), L-cystine (0.128g/L), bromothymol blue (0.02g/L), and agar (15g/L), with a final pH of 7.3 0.2 at 25C, appearing green when prepared. Key Specifications:

• Product Name: C.L.E.D. Agar w/ Bromo Thymol Blue
• Product Code: M792
• Intended Use: Isolation, enumeration, and identification of urinary pathogens.
• Appearance (Powder): Cream to yellow, homogeneous, free-flowing.
• Appearance (Prepared Medium): Green, clear to slightly opalescent gel.
• pH (Final): 7.3 0.2 (at 25C).
• Composition (per Liter):
  • Peptone: 4.0 g
  • Tryptone: 4.0 g
  • HM Peptone B: 3.0 g
  • Lactose: 10.0 g
  • L-Cystine: 0.128 g
  • Bromothymol Blue: 0.02 g
  • Agar: 15.0 g
• Directions: Suspend 36.15g in 1L water, boil to dissolve, sterilize (121C, 15 min), cool to 45-50C, pour plates.
• Storage: Store at 10-30C in a tightly closed container; keep dry. 

How it Works:

• Lactose Fermentation: Lactose is fermented by some bacteria, producing acid which turns the bromothymol blue indicator yellow.
• Cystine: Supports the growth of dwarf coliforms, crucial for urinary diagnostics.
• Appearance of Colonies: Blue/purple (non-fermenters like Proteus) or yellow (lactose fermenters like E. coli).