Cooked Meat Medium (R.C.Medium) Himedia 500g

About Cooked Meat Medium (R.C.Medium) Himedia 500g

• Cultivation: Ideal for Clostridium species (like C. welchii) and other aerobes/anaerobes.
• Reducing Environment: Contains cooked beef heart and glutathione, which create low oxidation-reduction potential (redox) for anaerobes to thrive.
• Nutrient Rich: Beef heart provides essential amino acids and growth factors.
• Stock Culture Maintenance: Used to preserve bacterial cultures.
• Proteolytic Activity: Helps determine if anaerobes can break down protein (indicated by meat blackening/disintegration). 

• Beef Heart Infusion: 500g
• Peptic Digest of Animal Tissue: 5g
• Sodium Chloride: 2.5g
• Final pH: 7.8 0.2 

1. Meat Particles: The cooked meat provides a surface and nutrients, while its denatured proteins offer available sulfhydryl groups, creating the necessary reducing conditions.
2. Glutathione: Acts as a powerful reducing agent, scavenging oxygen and allowing strict anaerobes to grow.
3. Dextrose (in some formulations): Allows for rapid growth and detection of anaerobes. 

• Prepare by suspending powder in distilled water, mixing, letting it wet, and autoclaving.
• Best used fresh; if stored, reheat gently (steam/boil) and cool without agitation before inoculation.
• Growth appears as turbidity or gas bubbles; blackening/disintegration signals proteolysis.