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Luria Bertani Broth Miller 100g Himedia

Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia

Luria Bertani Broth Miller 100g Himedia Specification

  • Boiling point
  • N/A
  • Purity
  • N/A (not applicable)
  • Packaging Type
  • Plastic Bottle
  • Molecular Weight
  • N/A formulated mixture Carat
  • Usage
  • Laboratory
  • Size
  • 100g
  • Shelf Life
  • 5 Years
  • Density
  • Not specified Gram per cubic centimeter(g/cm3)
  • Ph Level
  • 7.5 0.2
  • Flash Point
  • N/A
  • Physical State
  • Powder
  • Molecular Formula
  • N/A formulated mixture
  • Melting Point
  • N/A
  • CAS No
  • N/A formulated mixture
  • Grade
  • Dehydrated microbiological culture medium
  • Type
  • Other
  • Application
  • Laboratory
  • Appearance
  • Cream to yellow, homogeneous free-flowing powder
  • Purity(%)
  • N/A (not applicable)
  • Product Code
  • M1245
  • HSN CODE
  • 38220000
 

Luria Bertani Broth Miller 100g Himedia Trade Information

  • Minimum Order Quantity
  • 25 Kilograms
  • Supply Ability
  • 100 Kilograms Per Month
  • Delivery Time
  • 7 Days
 

Luria Bertani Broth Miller 100g Himedia About

Luria Bertani Broth, Miller (LB Miller) is a widely used, nutritionally rich culture medium in microbiology, particularly for growing Escherichia coli (E. coli) and other non-fastidious microorganisms. It's a key component in molecular biology for maintaining and cultivating recombinant strains of E. coli. The "Miller" designation indicates a specific formulation with a higher salt concentration compared to the Lennox modification of LB broth. Here's a more detailed breakdown:
  • Composition:LB Miller typically contains tryptone, yeast extract, and sodium chloride (NaCl). The Miller formulation has 10 g/L of NaCl, while the Lennox modification has 5 g/L. 
  • Purpose:LB Miller is designed to support the rapid growth of E. coli and is often used in molecular biology for:
    • Cultivating and maintaining E. coli strains, especially those engineered for genetic studies. 
    • Isolating and cultivating other non-fastidious microorganisms. 
    • Preparing bacterial cultures for various molecular biology techniques. 
  • Key Features:
    • Nutrient-rich: Provides essential amino acids, nucleotide precursors, vitamins, and other metabolites for robust bacterial growth. 
    • High salt concentration: The higher NaCl content is particularly useful for maintaining plasmids in E. coli cells. 
    • Versatile: Can be used in both liquid and agar plate formats (LB agar). 
  • Preparation:Typically, 25 grams of LB Miller powder is dissolved in 1 liter of distilled or purified water, gently heated to dissolve, and then autoclaved. 
  • Applications:LB Miller is extensively used in molecular biology, including:
    • Bacterial transformation experiments. 
    • Plasmid propagation and DNA cloning. 
    • Protein expression studies. 
    • Bacterial genetics research. 
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
Luria Bertani Broth Miller 100g Himedia
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