Mannitol Salt Agar Base 100g Himedia

Mannitol Salt Agar Base 100g Himedia

Mannitol Salt Agar Base 100g Himedia

Mannitol Salt Agar Base 100g Himedia Specification

Purity
N/A culture medium

Flash Point
not specified

Boiling point
N/A

Packaging Type
Plastic Bottle

Density
Not specified by HiMedia Gram per cubic centimeter(g/cm3)

Shelf Life
5 Years

CAS No
N/A complex mixture

Ph Level
7.4 0.2

Physical State
Powder

Usage
Laboratory

Molecular Weight
N/A Carat

Melting Point
N/A

Molecular Formula
N/A

Size
100g

Grade
Microbiological culture medium

Type
Industrial Lab Chemicals

Application
Laboratory

Appearance
Light yellow to pink, homogeneous, free-flowing powder

Purity(%)
N/A culture medium

Mannitol Salt Agar Base 100g Himedia Trade Information

Minimum Order Quantity
25 Pieces

Payment Terms
Cash in Advance (CID)

Supply Ability
5000 Pieces Per Month

Delivery Time
5 Days

Main Domestic Market
All India

Mannitol Salt Agar Base 100g Himedia About

HiMedia Mannitol Salt Agar (MSA) base is a selective and differential medium used to isolate and identify staphylococci, particularly Staphylococcus aureus. It's a medium recommended for selecting and subculturing S. aureus in accordance with the harmonized methods of USP/EP/BP/JP/IP according to HiMedia. High salt concentrations inhibit other bacteria, and mannitol fermentation, indicated by phenol red, aids in the differential identification. Key Features:

Selective:The high salt concentration (7.5% NaCl) inhibits the growth of most non-staphylococcal bacteria, allowing for selective isolation of Staphylococci.
Differential:Mannitol fermentation, detected by the phenol red indicator, differentiates Staphylococcus aureus (which ferments mannitol, producing yellow colonies) from other staphylococci that may or may not ferment mannitol.
Application:Used for the detection and enumeration of coagulase-positive Staphylococci in various samples like milk, food, and clinical specimens.
Compliance:The medium's composition complies with specifications in Chapter 62 of the U.S. Pharmacopoeia (USP) related to microbial limit testing.

How it Works:

Staphylococci that can ferment mannitol will produce acidic byproducts, lowering the pH of the medium.
The acidic environment causes the phenol red indicator to turn yellow around the colonies.
S. aureus typically exhibits yellow colonies with a halo, while other staphylococci may have different appearances.

Indications:

This medium is particularly useful for the detection and enumeration of S. aureus in various samples.
It can be used for routine isolation and subculture of staphylococci.
The medium is recommended for use in microbial limit testing according to the harmonized USP/EP/BP/JP/IP methods.

Mannitol Salt Agar Base 100g Himedia

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