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Mannitol Salt Agar Base 100g Himedia

Mannitol Salt Agar Base 100g Himedia
Mannitol Salt Agar Base 100g Himedia

Mannitol Salt Agar Base 100g Himedia Specification

  • Purity
  • N/A culture medium
  • Molecular Weight
  • N/A Carat
  • Molecular Formula
  • N/A
  • Flash Point
  • not specified
  • Physical State
  • Powder
  • Size
  • 100g
  • Packaging Type
  • Plastic Bottle
  • Shelf Life
  • 5 Years
  • Usage
  • Laboratory
  • Ph Level
  • 7.4 0.2
  • Density
  • Not specified by HiMedia Gram per cubic centimeter(g/cm3)
  • Melting Point
  • N/A
  • CAS No
  • N/A complex mixture
  • Boiling point
  • N/A
  • Grade
  • Microbiological culture medium
  • Type
  • Industrial Lab Chemicals
  • Application
  • Laboratory
  • Appearance
  • Light yellow to pink, homogeneous, free-flowing powder
  • Purity(%)
  • N/A culture medium
 

Mannitol Salt Agar Base 100g Himedia Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • Payment Terms
  • Cash in Advance (CID)
  • Supply Ability
  • 5000 Pieces Per Month
  • Delivery Time
  • 5 Days
  • Main Domestic Market
  • All India
 

About Mannitol Salt Agar Base 100g Himedia

HiMedia Mannitol Salt Agar (MSA) base is a selective and differential medium used to isolate and identify staphylococci, particularly Staphylococcus aureus. It's a medium recommended for selecting and subculturing S. aureus in accordance with the harmonized methods of USP/EP/BP/JP/IP according to HiMedia. High salt concentrations inhibit other bacteria, and mannitol fermentation, indicated by phenol red, aids in the differential identification. Key Features:
  • Selective:The high salt concentration (7.5% NaCl) inhibits the growth of most non-staphylococcal bacteria, allowing for selective isolation of Staphylococci. 
  • Differential:Mannitol fermentation, detected by the phenol red indicator, differentiates Staphylococcus aureus (which ferments mannitol, producing yellow colonies) from other staphylococci that may or may not ferment mannitol. 
  • Application:Used for the detection and enumeration of coagulase-positive Staphylococci in various samples like milk, food, and clinical specimens. 
  • Compliance:The medium's composition complies with specifications in Chapter 62 of the U.S. Pharmacopoeia (USP) related to microbial limit testing. 
How it Works:
  1. Staphylococci that can ferment mannitol will produce acidic byproducts, lowering the pH of the medium. 
  2. The acidic environment causes the phenol red indicator to turn yellow around the colonies. 
  3. S. aureus typically exhibits yellow colonies with a halo, while other staphylococci may have different appearances. 
Indications:
  • This medium is particularly useful for the detection and enumeration of S. aureus in various samples. 
  • It can be used for routine isolation and subculture of staphylococci. 
  • The medium is recommended for use in microbial limit testing according to the harmonized USP/EP/BP/JP/IP methods. 
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