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Plate Count Agar (Standard Methods Agar) 500g Himedia

Plate Count Agar (Standard Methods Agar) 500g Himedia
Plate Count Agar (Standard Methods Agar) 500g Himedia
Plate Count Agar (Standard Methods Agar) 500g Himedia
Plate Count Agar (Standard Methods Agar) 500g Himedia
Plate Count Agar (Standard Methods Agar) 500g Himedia
Plate Count Agar (Standard Methods Agar) 500g Himedia

Plate Count Agar (Standard Methods Agar) 500g Himedia Specification

  • Physical State
  • Powder
  • Packaging Type
  • Bottle
  • Size
  • Bottle of 100gm
  • Usage
  • Chemical Laboratory
  • Product Code
  • M091
 

Plate Count Agar (Standard Methods Agar) 500g Himedia Trade Information

  • Minimum Order Quantity
  • 20 Pieces
  • Supply Ability
  • 100 Pieces Per Month
  • Delivery Time
  • 7 Days
 

About Plate Count Agar (Standard Methods Agar) 500g Himedia

Plate Count Agar (PCA), also known as Standard Methods Agar, is a non-selective, general-purpose microbiological growth medium used to determine the number of viable microorganisms (primarily bacteria) in a sample. It's commonly used to assess the total microbial load in foods, water, and other materials of sanitary importance. Here's a more detailed breakdown:Purpose and Usage:
  • Enumeration of Microorganisms: PCA is used to count the number of viable bacteria, yeasts, and molds in a sample, typically by the pour plate or spread plate method. 
  • Quality Control: It's a standard tool for monitoring microbial contamination in various industries, including food production, water treatment, and clinical settings. 
  • Testing: PCA is used in testing for microbial contamination in food and water. 
Key Features:
  • Non-selective:PCA doesn't contain any ingredients that inhibit or favor the growth of specific microorganisms. This means it allows a wide range of microorganisms to grow, providing a general count of the viable microbial population. 
  • Nutrient-rich:The medium contains nutrients like peptone, yeast extract, and dextrose, which provide the necessary carbon and nitrogen sources for microbial growth. 
  • Standard Formulation:The formula is based on the Buchbinder et al. method, which is recommended by the American Public Health Association (APHA) and the Food and Drug Administration (FDA) for determining plate counts. 
How it's used:
  1. 1. Sample Preparation:A sample (e.g., food, water) is diluted using sterile saline to obtain a manageable number of microorganisms for plating. 
  2. 2. Plating:The diluted sample is then plated onto PCA plates. This can be done by either the pour plate method (where molten agar is mixed with the sample) or the spread plate method (where a small amount of the sample is spread on the surface of solidified agar). 
  3. 3. Incubation:The plates are incubated at a specific temperature (typically 32-37C for bacteria) for a set period (usually 24-48 hours). 
  4. 4. Colony Counting:After incubation, individual colonies (each representing a single microbial cell or group of cells) are counted on the plates. 
  5. 5. Interpretation:The number of colonies is used to calculate the number of microorganisms per unit volume or weight of the original sample. 
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