Simmons Citrate Agar 100g Himedia

Simmons Citrate Agar 100g Himedia

Simmons Citrate Agar 100g Himedia

Simmons Citrate Agar 100g Himedia Specification

Boiling point
N/A mixture

Density
N/A / not specified by manufacturer Gram per cubic centimeter(g/cm3)

Purity
Not applicable as a single % purity

Molecular Formula
N/A mixture

Molecular Weight
N/A mixture Grams (g)

CAS No
N/A mixture

Shelf Life
5 Years

Packaging Type
Plastic Bottle

Melting Point
N/A mixture

Ph Level
6.8 0.2

Usage
Laboratory

Size
100g

Flash Point
N/A / not applicable

Physical State
Solid

Grade
Microbiological / Culture Media Grade

Type
Industrial Lab Chemicals

Application
Laboratory

Appearance
Cream to yellow, homogeneous, free-flowing powder

Purity(%)
Not applicable as a single % purity

Simmons Citrate Agar 100g Himedia Trade Information

Minimum Order Quantity
20 Pieces

Supply Ability
4000 Pieces Per Month

Delivery Time
5 Days

Main Domestic Market
All India

Simmons Citrate Agar 100g Himedia About

Simmons Citrate Agar is a specialized culture medium used to differentiate bacteria, particularly Gram-negative bacteria, based on their ability to utilize citrate as their sole carbon source. The medium contains sodium citrate as the carbon source and ammonium salts as the sole nitrogen source. A positive result is indicated by a color change from green to blue, due to the alkaline byproducts of citrate metabolism. Here's a more detailed breakdown:Composition and Principle:

Defined Medium:Simmons Citrate Agar is a defined medium, meaning its exact chemical composition is known.
Carbon and Nitrogen Source:It contains sodium citrate as the sole carbon source and ammonium salts as the sole nitrogen source.
pH Indicator:Bromothymol blue is included as a pH indicator, changing from green to blue as the medium becomes alkaline.
Citrate Utilization:Bacteria capable of using citrate will metabolize it, producing alkaline byproducts that raise the pH of the medium.
Selective and Differential:The medium is both selective and differential. It selects for bacteria that can utilize citrate and differentiates them based on their ability to do so.

How it works:

Inoculation: A bacterial sample is streaked onto the slant of the Simmons Citrate Agar.
Incubation: The inoculated medium is incubated at 35-37C for 18-24 hours.
Observation:
- Positive Result: A blue color change indicates that the bacteria has metabolized the citrate, producing alkaline byproducts.
- Negative Result: A green color indicates that the bacteria was unable to utilize citrate as a carbon source.

Uses:

Differentiation of Bacteria:It is primarily used to differentiate between various Gram-negative bacteria, especially within the Enterobacteriaceae family, based on their citrate utilization.
IMViC Tests:Simmons Citrate Agar is a component of the IMViC tests, a battery of biochemical tests used to identify bacteria.
Clinical Microbiology:It is used in clinical microbiology laboratories to identify and classify bacterial

Simmons Citrate Agar 100g Himedia

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