Antibiotic Assay Medium No 11 500g Himedia

Antibiotic Assay Medium No. 11, also known as Neomycin, Erythromycin Assay Agar, is a specialized medium used for microbiological assays of antibiotics. It's designed to support the growth of specific test organisms while allowing for the measurement of antibiotic activity. The medium is particularly useful for assays of antibiotics like neomycin and erythromycin, and others. Key Features and Uses:

• Specific Antibiotic Assays:This medium is specifically designed for assays of various antibiotics, including neomycin, erythromycin, chlortetracycline, and others.
• Microbiological Assay:It's used in microbiological assays to determine the potency and activity of antibiotics.
• Test Organisms:It supports the growth of specific test organisms, such as Bacillus pumilis, Bacillus subtilis, and Micrococcus luteus, which are used to determine antibiotic activity.
• pH Control:The medium is typically prepared to have a pH of 8.3 +/- 0.2.
• Seed Agar:It can also be used as a seed layer or base layer in various assay procedures. 

Preparation and Usage:

• Preparation:The medium is prepared according to specific instructions, typically involving adding the dehydrated powder to distilled water and autoclaving. 
• Inoculation:Test organisms are inoculated onto the agar surface after it has been cooled. 
• Antibiotic Diffusion:Antibiotics are then introduced to the medium, and their diffusion through the agar is monitored for zones of inhibition, which indicate antibiotic activity. 

In summary, Antibiotic Assay Medium No. 11 is a specialized medium used for microbiological assays of various antibiotics, particularly neomycin and erythromycin, and allows for the determination of antibiotic potency and activity through the observation of zones of inhibition