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Bile Esculin 1vl

Bile Esculin 1vl
Bile Esculin 1vl
Bile Esculin 1vl
Bile Esculin 1vl
Bile Esculin 1vl
Bile Esculin 1vl

Bile Esculin 1vl Specification

  • Ingredients
  • specific nutrients, selective agents, and indicators per liter of purified water
  • Product Type
  • Biochemical identification test discs
  • Properties
  • Bile Esculin Agar, a product by HiMedia, is a selective and differential medium used for the isolation and presumptive identification of Group D Streptococci and Enterococci, particularly from food and pharmaceutical samples. It is based on the ability of these bacteria to hydrolyze esculin in the presence of bile. Here's a more detailed description
  • Purity
  • high-purity laboratory-grade components intended for selective microbiological identification
  • Melting Point
  • Not applicable (powder mixture)
  • EINECS No
  • 208-517-5
  • Density
  • 1.02 Gram per cubic centimeter(g/cm3)
  • Appearance
  • Homogeneous free-flowing beige to light brown powder
  • Usage
  • Microbiological assay, culture media for laboratory testing
  • Smell
  • Other
  • Taste
  • Other
  • Physical Form
  • Powder, Solid
  • Grade
  • Laboratory or microbiological grade
  • Shape
  • other
  • Storage
  • Other
  • CAS No
  • 531-75-9
  • Molecular Weight
  • N/A (Mixture) Grams (g)
  • Molecular Formula
  • N/A (Mixture)
  • Application
  • Other
  • Ph Level
  • 7.1 0.2 at 25 C (prepared medium)
  • Poisonous
  • YES
  • Solubility
  • Soluble in water
  • Shelf Life
  • 3 Years
  • Structural Formula
  • N/A (mixture, not a single compound)
  • HS Code
  • 38220000
  • Hazard Statement
  • Not classified as hazardous under GHS
  • Lot Number
  • Indicated on product label
  • Indicator
  • Esculin hydrolysis detected by blackening of the medium
  • Pack Size
  • Varies commonly 500 g or 100 g bottles available
  • pH after Sterilization
  • 7.1 0.2 at 25C
  • Manufacturing Date
  • Indicated on product packaging
  • Disposal
  • Dispose of all used material as per laboratory biosafety guidelines
  • Preparation Directions
  • Suspend the recommended quantity of powder in distilled water, heat to dissolve, distribute into containers and sterilize by autoclaving at 121C for 15 minutes
  • Quality Control
  • Tested for ability to promote growth of Enterococcus faecalis and inhibit non-group D bacteria
 

Bile Esculin 1vl Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • FOB Port
  • Jaipur
  • Payment Terms
  • Cash Advance (CA), Cash in Advance (CID), Telegraphic Transfer (T/T)
  • Supply Ability
  • 100 Pieces Per Day
  • Delivery Time
  • 5 Days
  • Sample Policy
  • Contact us for information regarding our sample policy
  • Main Export Market(s)
  • Asia, South America, Australia, Central America, North America, Eastern Europe, Western Europe, Africa, Middle East
  • Main Domestic Market
  • All India
 

Bile Esculin 1vl About

Bile Esculin Agar, a product by HiMedia, is a selective and differential medium used for the isolation and presumptive identification of Group D Streptococci and Enterococci, particularly from food and pharmaceutical samples. It is based on the ability of these bacteria to hydrolyze esculin in the presence of bile. Here's a more detailed description:Key Features:
  • Selective:Bile inhibits the growth of many bacteria, allowing for the isolation of bile-tolerant organisms like Group D Streptococci and Enterococci. 
  • Differential:The medium contains esculin, which is hydrolyzed by these bacteria into esculetin and dextrose. 
  • Indicator:Esculetin reacts with ferric citrate, producing a characteristic black precipitate, indicating a positive result. 
  • Applications:Commonly used in food and pharmaceutical microbiology for:
    • Isolation and identification of Group D Streptococci. 
    • Differentiation of Enterococci from other bacteria. 
    • Detection of Yersinia enterocolitica, the causative agent of Yersiniosis. 
Mechanism:
  1. 1. Bile Tolerance:Bile salts inhibit the growth of many microorganisms, but certain bacteria like Enterococci and Group D Streptococci are bile-tolerant and can grow on this medium. 
  2. 2. Esculin Hydrolysis:These bacteria possess the enzyme esculinase, which breaks down esculin into esculetin and dextrose. 
  3. 3. Colorimetric Detection:Dextrose is then metabolized, and the resulting esculetin reacts with ferric citrate (present in the medium) to form a black precipitate, indicating esculin hydrolysis. 
Interpretation:
  • Positive Result: Blackening of the medium around the bacterial colonies indicates esculin hydrolysis and a positive test for Group D Streptococci or Enterococci.
  • Negative Result: Lack of blackening suggests that the bacteria do not hydrolyze esculin. 
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