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Barritt Reagent B (for VP test) 100ml Himedia

Barritt Reagent B (for VP test) 100ml Himedia
Barritt Reagent B (for VP test) 100ml Himedia
Barritt Reagent B (for VP test) 100ml Himedia
Barritt Reagent B (for VP test) 100ml Himedia
Barritt Reagent B (for VP test) 100ml Himedia
Barritt Reagent B (for VP test) 100ml Himedia

Barritt Reagent B (for VP test) 100ml Himedia Specification

  • Size
  • 100 ml
  • Packaging Type
  • Plastic Bottle
  • Usage
  • Laboratory
  • Grade
  • LR (Laboratory)
  • Colorless Solution
  • Yes
  • For VP Test
  • Yes
  • Reagent Type
  • Barritt B
  • Ready to Use
  • Yes
 

Barritt Reagent B (for VP test) 100ml Himedia Trade Information

  • Minimum Order Quantity
  • 25 Pieces
  • Supply Ability
  • 100 Pieces Per Month
  • Delivery Time
  • 7 Days
 

About Barritt Reagent B (for VP test) 100ml Himedia

HiMedia's Barritt Reagent B is a 40% potassium hydroxide solution used in the Voges-Proskauer (VP) test to detect the production of acetoin by bacteria. It works in conjunction with Barritt Reagent A (alpha-naphthol) to produce a red color, indicating that a bacterium has fermented glucose to produce neutral end products like acetoin. The VP test, which uses these reagents with cultures grown in MR-VP Medium, is a biochemical test for identifying members of the Enterobacteriaceae family. Function in the VP Test
  • Acetoin Detection:The VP test identifies bacteria that produce neutral fermentation end products, such as acetoin. 
  • Color Reaction:In the presence of oxygen, acetoin (from the culture) is oxidized and reacts with the alpha-naphthol and potassium hydroxide from the Barritt's reagents to form a red or cherry-red complex. 
Composition
  • Barritt Reagent B is a 40% potassium hydroxide (KOH) solution. 
  • It is part of the Barritt's reagent set, with Reagent A being the alpha-naphthol component. 
Application
  • The test is performed on 24-48 hour old bacterial cultures grown in MR-VP broth. 
  • A specific volume of Reagent B is added to the culture medium along with Reagent A. 
Interpretation
  • A positive VP test is indicated by the formation of a red color within 5-10 minutes after mixing the reagents and gently shaking to expose the medium to air. 
  • A negative test shows no red color change. 
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